Hypoxic pulmonary vasoconstriction (HPV) matches lung perfusion with ventil
ation. Controversy exists whether decreased or increased reactive oxygen sp
ecies may elicit HPV and from which source such oxygen metabolites are deri
ved. In rabbit lungs, we detected transcripts of a nonphagocytic NADPH oxid
ase subunit homologous to mitogenic oxidase-1 (Mox1) or NADPH oxidase homol
og 1 (NOH-1L). In perfused rabbit lungs, we employed 1) a new NADPH oxidase
inhibitor [4-( 2-aminoethyl)benzenesulfonyl fluoride (AEBSF; 100-600 mu M)
] and 2) the superoxide dismutase (SOD) inhibitors diethyldithiocarbamic ac
id (DETC; 100 mu M to 10 mM) and triethylenetetramine (TETA; 1-25 mM). Spec
ificity of these agents for HPV was investigated by comparison with U-46619
-induced vasoconstrictions. AEBSF induced a transient increase in pulmonary
arterial pressure with increased strength of HPV. Subsequent to this initi
al response, normoxic pulmonary arterial pressure was not affected and HPV
was specifically suppressed. Whereas DETC turned out to act in a nonspecifi
c fashion, TETA suppressed HPV specifically. These findings provide evidenc
e of a role for a nonphagocytic NAD(P)H oxidase with superoxide and SOD-rel
ated hydrogen peroxide formation in HPV. Because HPV was inhibited but not
mimicked by the inhibitors, increased rather than decreased superoxide and/
or hydrogen peroxide formation is suggested as the hypoxia-provoked signali
ng event.