Chemiluminescent determination of Ce(IV) using Cypridina luciferin analog

A cerium(IV)-induced Cypridina luciferin analog (CLA) chemiluminescence is reported. This Ce(IV) triggered luciferin analog CL is enhanced about 5-fol d by 0.5% (v/v) Tween 20. Using this enhanced luciferin analog-dependent ch emiluminescence, a lower limit detection of 25 nM was determined for Ce(IV) . Ce(III) could not generate this luciferin analog-dependent chemiluminesce nce. Oxidising agents such as potassium permanganate and potassium dichroma te were also without effect. While the tested scavengers of reactive specie s such as mannitol, superoxide dismutase and catalase had no specific effec t on the luminescence, this Ce(IV)-elicited luciferin analog chemiluminsece nt reaction was inhibited when the reaction was performed under anaerobic c ondition, indicating that molecular oxygen participates in the reaction mec hanism leading to the formation of the light-emitting product. A mechanism is proposed for this chemiluminescent reaction which is consistent with the presented data. (C) 2000 Elsevier Science B.V. All rights reserved.