Linker-mediated modulation of the chemiluminescent signal from N-10-(3-sulfopropyl)-N-sulfonylacridinium-9-carboxamide tracers

Authors
Adamczyk, M Chen, YY Fishpaugh, JR Mattingly, PG Pan, Y Shreder, K Yu, ZG
Citation
M. Adamczyk et al., Linker-mediated modulation of the chemiluminescent signal from N-10-(3-sulfopropyl)-N-sulfonylacridinium-9-carboxamide tracers, BIOCONJ CHE, 11(5), 2000, pp. 714-724
Citations number
36
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOCONJUGATE CHEMISTRY
ISSN journal
10431802 → ACNP
Volume
11
Issue
5
Year of publication
2000
Pages
714 - 724
Database
ISI
SICI code
1043-1802(200009/10)11:5<714:LMOTCS>2.0.ZU;2-J
Abstract
Four chemiluminescent N-sulfonylacridinium-9-carboxamide active esters (17- 20) were prepared from the corresponding acids and coupled to bath of the a minated phenobarbital (13) and N-(6-aminohexyl)phenytoin (16) haptens. The level of signal produced by chemiluminescent N- sulfonylacridinium-9-carbox amide phenobarbital and phenytoin tracers in a solid-phase immunoassay form at was found to be modulated by at least 20-fold by the judicious choice of the reactive acridinium-hapten linking group.