Jj. Gooding et al., Parameters important in tuning the response of monolayer enzyme electrodesfabricated using self-assembled monolayers of alkanethiols, BIOSENS BIO, 15(5-6), 2000, pp. 229-239
Enzyme electrodes were observed experimentally to have a broad dynamic rang
e, high sensitivity and excellent reproducibility. The theoretically predic
ted response of the monolayer enzyme electrodes was in good agreement with
that observed experimentally over the broad range of experimental condition
s tested. The response is limited by the rate of enzyme turnover by a media
ting species rather than mass transport. As a consequence of this limitatio
n, the response was very sensitive to the enzyme loading and the concentrat
ion of mediator in the sample solution but insensitive to mass transport va
riables such as solution stirring or the diffusion coefficients of the subs
trate or cosubstrate. (C) 2000 Elsevier Science S.A. All rights reserved.