Glutathione depletion inhibits IL-1 beta-stimulated nitric oxide production by reducing inducible nitric oxide synthase gene expression

Authors
Nikulina, MA Andersen, HU Karlsen, AE Darville, MI Eizirik, DL Mandrup-Poulsen, T
Citation
Ma. Nikulina et al., Glutathione depletion inhibits IL-1 beta-stimulated nitric oxide production by reducing inducible nitric oxide synthase gene expression, CYTOKINE, 12(9), 2000, pp. 1391-1394
Citations number
15
Categorie Soggetti
Cell & Developmental Biology
Journal title
CYTOKINE
ISSN journal
10434666 → ACNP
Volume
12
Issue
9
Year of publication
2000
Pages
1391 - 1394
Database
ISI
SICI code
1043-4666(200009)12:9<1391:GDIIBN>2.0.ZU;2-5
Abstract
L-buthionine-S,R-sulfoximine (BSO), an inhibitor of GSH synthesis, decrease d IL-1 beta-induced nitrite release in rat islets and purified rat beta cel ls, nitrite formation and iNOS gene promoter activity in insulinoma cells, and iNOS mRNA expression in rat islets. The thiol depletor diethyl maleate (DEM) and an inhibitor of glutathione reductase 1,3-bis(2-chloroethyl)-1-ni trosourea (BCNU) reduced IL-1 beta-stimulated nitrite release in islets. We conclude that GSH regulates IL-1 beta-induced NO production in islets, pur ified beta cells and insulinoma cells by modulation of iNOS gene expression . (C) 2000 Academic Press.