Inter embryonic (homo- or hetero-sexual) transfer of primordial germ cellsbetween chicken embryos

Chicken primordial germ cells (PGCs) collected from the circulating blood i n embryonic vessels at stage 13-15 were inter-embryonically, homo- or heter o-sexually, transferred to the blood vessels of recipient embryos at the sa me stage of development. Approximately 30% of the embryos treated with hete ro-sexual transfer of PGCs had abnormal gonads, showing ovotestis like orga ns. In this case, some of these reversed gonads were considered to be depen dent upon the ratio of the number of PGCs from donor to recipient embryos. One of the treated embryos possessed completely reversed organs. Therefore, the introduction of exogenous embryonic vessels was thought to be also use ful for producing transgened gonads.