Prediction of the coding sequences of unidentified human genes. XVIII. Thecomplete sequences of 100 new cDNA clones from brain which code for large proteins in vitro

In our series of human cDNA projects for accumulating sequence information on the coding sequences of unidentified genes, we herein present the entire sequences of 100 cDNA clones of unidentified genes, named KIAA1544 to KIAA 1643, from two sets of size-fractionated human adult and fetal brain cDNA l ibraries. The average sizes of the inserts and corresponding open reading f rames of cDNA clones analyzed here reached 4.6 kb and 2.8 kb (930 amino aci d residues): respectively. By computer-assisted database search of the dedu ced amino acid sequences, 48 predicted gene products were classified into t he five functional categories of proteins relating to cell signaling/commun ication, nucleic acid management, cell structure/motility, protein manageme nt and metabolism. Homology search against the databases for proteins deduc ed from yeast, nematode and fly full genome sequences revealed only one gen e (KIAA1630) was entirely conserved among human and these three organisms i n the 100 genes reported here. Additionally, their chromosomal loci were de termined by using human-rodent hybrid panels unless they were already assig ned in the public databases. Furthermore, the expression profiles of the ge nes were also studied in 10 human tissues: 8 brain regions, spinal cord, fe tal brain and fetal liver by reverse transcription-coupled polymerase cl la in reaction, products of which were quantified by enzyme-linked immunosorbe nt assay.