Prediction of the codling sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro

We have carried out a human cDNA sequencing project to accumulate informati on regarding the coding sequences of unidentified human genes. As an extens ion of the preceding reports, we herein present the entire sequences of 150 cDNA clones of unknown human genes, named KIAA1294 to KIAA1443, from two s ets of size-fractionated human adult and fetal brain cDNA libraries. The av erage sizes of the inserts and corresponding open reading frames of cDNA cl ones analyzed here reached 4.8 kb and 2.7 kb (910 amino acid residues), res pectively. From sequence similarities and protein motifs, 73 predicted gene products were functionally annotated and 97% of them were classified into the following four functional categories: cell signaling/communication, nuc leic acid management, cell structure/motility and protein management. Addit ionally, the chromosomal loci of the genes were assigned by using human-rod ent hybrid panels for those genes whose mapping data were not available in the public databases. The expression profiles of the genes were also studie d in 10 human tissues, 8 brain regions, spinal cord, fetal brain and fetal liver by reverse transcription-coupled polymerase chain reaction, products of which were quantified by enzyme-linked immunosorbent assay.