Analysis of the domain requirement in Gas1 growth suppressing activity

The product of the growth arrest specific gene, gas1, is a membrane-associa ted protein which activates a p53-dependent growth suppression signalling p athway. We have shown that Gas1 is linked to the plasma membrane through a glycosyl-phosphatidylinositol (GPI) anchor, Several GPI-anchored protein ha ve been identified as part of receptor complexes either as co-receptors or as membrane bound ligands. In this report, we characterize the Gas1 domains required for its growth suppression function and demonstrate the dispensab ility of Gas1 GPI anchor. (C) 2000 Federation of European Biochemical Socie ties. Published by Elsevier Science B.V. All rights reserved.