Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase cDNA of Japanese flounder Paralichthys olivaceus

Japanese flounder Paralichthys olivaceus glyceraldehyde-3-phosphate dehydro genase (GAPDH or G3PD) cDNA was cloned and sequenced. Japanese flounder GAP DH was found to consist of 333 amino acid residues and to exhibit a high de gree of homology to previously reported GAPDH of vertebrates, in both the D NA and amino acid sequences. Curiously, Japanese flounder GAPDH was found t o be more homologous to GAPDH of Xenopus, chicken and mammals than to that of rainbow trout. We developed primers for RT-PCR and used this method to a mplify GAPDH mRNA from various tissues to determine whether it could be use d as an internal standard. The GAPDH was expressed in all organs and tissue s, and confirmed that it represents a good internal standard positive contr ol for the study of gene expression.