The 1998-1999 collaborative exercises and proficiency testing program on DNA typing of the Spanish and Portuguese Working Group of the International Society for Forensic Genetics (GEP-ISFG)

A total of 28 laboratories (labs) submitted results for the 1998 collaborat ive exercise and the proficiency testing program of the Spanish and Portugu ese Working Group of the International Society for Forensic Genetics (CEP-I SFG) group. This number increased to 46 labs in 1999. Six bloodstains were submitted, each one with 200 mu l soaked in cotton except the sample no. 6 submitted for DNA quantification which had 2 mu l. One of the samples was a mixed stain. A paternity testing case and a criminal case in the 1998 tria l (GEP'98) and two paternity testing cases in 1999 (GEP'99) were included a nd the statistical evaluation of the evidence was requested in both cases. In the GEP'99 trial, a theoretical paternity testing case was included. A t otal of 52 DNA genetic markers were used by the participants in the GEP'98 trial, which increased to 101 in GEP'99. Despite this increasing number of participating labs, results remained quite satisfactory. All the labs used PCR-based DNA polymorphisms with an increasing number of markers, obtaining good results. SLPs were used by a decreasing number of labs but the result s indicated a good level of expertise despite the different protocols used. Good results were also obtained for mtDNA despite the difficulties presente d by the samples due to the presence of length heteroplasmy in some samples in both trials. The detection of heteroplasmy should, however, be improved . Similar conclusions were reached for both, the paternity and the criminal c ase by all the labs. Common methodologies for the statistical evaluation of the paternity case were used and the paternity index and the probability o f paternity (with an a priori value of 0.5) reported by most of the labs. A lso, a great uniformity was found in the evaluation of the criminal case de spite the lack of a specific hypothesis in the design of the exercise. Some errors in statistical programs or in calculations were detected in a theor etical paternity case included in the GEP'99 trial for statistical analysis . (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.