Development of a set of near isogenic and backcross recombinant inbred lines containing most of the Lycopersicon hirsutum genome in a L-esculentum genetic background: A tool for gene mapping and gene discovery
Aj. Monforte et Sd. Tanksley, Development of a set of near isogenic and backcross recombinant inbred lines containing most of the Lycopersicon hirsutum genome in a L-esculentum genetic background: A tool for gene mapping and gene discovery, GENOME, 43(5), 2000, pp. 803-813
A novel population consisted of a set of 99 near isogenic lines (NILs) and
backcross recombinant inbred lines (BCRILs) derived from a cross between th
e cultivated tomato Lycopersicon esculentum cv. E6206 and L. hirsutum acces
sion LA1777 is presented. Most of the lines contain a single defined introg
ression from L. hirsutum in the L. esculentum genetic background and togeth
er, the lines provide a coverage of more than the 85% of the L. hirsutum ge
nome. These lines represent a new tool to uncover the genetic resources hid
den in L. hirsutum as well as to study the genes responsible of its unique
biology. Furthermore, the study of the allelic frequency and heterozygosity
among BCRILs showed that specific genomic regions were likely subjected to
unintentional selection pressures during the stock development. Genes invo
lved in the reproductive behavior and (or) pollen viability are hypothesize
d to be responsible for these alterations.