Cloning and localization of C2orf2(ropp120), a previously unknown WD repeat protein

Ropp 120 (restrictedly overexpressed proliferation-associated protein) is a cytoplasmic protein of 120 kDa that is significantly overexpressed in mito tic cells. Protein sequencing of the immunoaffinity purified 120-kDa protei n showed it to be an as yet unknown protein. DNA sequencing revealed a cDNA sequence of 3419 bases, which includes the complete coding region of ropp1 20 of 2943 bases (981 amino acids). Analysis of the deduced amino acid sequ ence showed that ropp 120 contains four WD repeats and a well-conserved con sensus sequence of serine proteases. The gene encoding ropp120 (HGMW-approv ed gene symbol C2orf2) was assigned to chromosome 2p21-p22 by means of radi ation hybrid and fluorescence in situ hybridization mapping. (C) 2000 Acade mic Press.