Current estimates of plant availability of manure N are highly variable. A
study was conducted to examine C and N mineralization characteristics of ma
nure components from feeds (immature and mature alfalfa and orchardgrass, s
oybean meal, roasted soybean meal), forage cell walls (acid detergent fiber
[ADF] and neutral detergent fiber [NDF]), simple N compounds (urea, amino
acids and peptides), and nondietary metabolic components (ruminal bacteria
and colonic cells). Nitrogen and C mineralization were determined by aerobi
c incubation of sample-amended soil. Changes in NH4+ and NO3- concentration
and CO2 production were monitored over 112 d. At C to N ratios below 15, t
here was a declining exponential relationship between the initial potential
rate of C mineralization and the sample C to N ratio. However, at C to N r
atios above 15, the initial potential rate of C mineralization was low and
insensitive to C to N ratio. Percent of added N mineralized ranged from 92%
for urea and 13% for mature orchardgrass to a net N immobilization for ADF
- and NDF-amended soils. When the C to N ratio was below 42, there was a li
near relationship between readily mineralizable N and the C to N ratio. The
re were also positive relationships between readily mineralizable C and N a
nd the components' total N concentration. Manure contains a range of compou
nds that have rapid or intermediate N mineralization characteristics, or th
at are strong immobilizers of N. These results suggest that improved estima
tes of manure N mineralization may be obtained by considering both the read
ily available N components and components that strongly immobilize N.