Liquid chromatographic method for the determination of anthraquinone residues in weathered and unweathered formulated rice seed and surface water in rice fields

Anthraquinone was extracted from weathered and non-weathered formulated ric e seed and analyzed by reversed-phase high performance liquid chromatograph y. Anthraquinone was quantified by UV absorbance at 325 nm. Recovery data w ere determined by analyzing anthraquinone fortified control rice seed. The mean recovery of anthraquinone in rice seed was 99.3 +/- 0.5% for the range of 0.050 to 1.00% anthraquinone. Anthraquinone residues in surface water s amples from rice fields were determined by direct injection of filtered sam ples into a reversed-phase high performance liquid chromatograph and quanti fied by UV absorbance at 254 nm. Recovery data were produced by analyzing a nthraquinone fortified control surface water samples from rice fields. The mean recovery of anthraquinone in surface water samples was 97.0 +/- 2.4% f or the range of 8.0 to 400 ng/mL anthraquinone.