Jy. Nam et al., 8-O-methylsclerotiorinamine, antagonist of the Grb2-SH2 domain, isolated from Penicillium multicolor, J NAT PROD, 63(9), 2000, pp. 1303-1305
A new secondary metabolite, 8-O-methylsclerotiorinamine (1), was isolated f
rom a strain of Penicillium multicolor, and its structure was established u
sing NMR spectroscopy and chemical evidence. The metabolite inhibited signi
ficantly the binding between the Grb2-SH2 domain and the phosphopeptide der
ived from the Shc protein and also blocked the protein-protein interactions
of Grb2-Shc in cell-based experiments, with IC50 values of 5.3 and 50 mu M
, respectively.