INDICATOR CELL-LINES FOR DETECTION OF PRIMARY STRAINS OF HUMAN AND SIMIAN IMMUNODEFICIENCY VIRUSES

CCR5 and CXCR4 are the two major coreceptors that have been identified for human immunodeficiency virus (HIV) entry. We have modified severa l beta-galactosidase-based HIV indicator cell lines to express CCR5 an d/or CXCR4. Using these new reagents, we have been able to detect all primary isolates tested using one or both of these cell lines. However , there is large variation in the absolute viral infectivity among pri mary strains. Furthermore, all HIV strains are capable of causing sync ytia in the indicator cells when the coreceptor is present regardless of whether they had previously been characterized as ''syncytia-induci ng'' or ''non-syncytium-inducing.'' (C) 1997 Academic Press.