Matrix-assisted laser desorption/ionization mass spectrometry methods for oligodeoxynucleotides: Improvements in matrix, detection limits, quantification, and sequencing

A comatrix of anthranilic acid and nicotinic acid is optimum for the matrix -assisted laser desorption/ionization time of flight determination of oligo deoxynucleotides that are comprised of up to 21 nucleotides. A detection li mit of approximately 200 amol was obtained for an oligonucleotide 21mer. Th e comatrix system is also suitable for quantification of oligodeoxynucleoti des provided an internal standard having one more or less nucleotide than t he number in the analyte is used. Furthermore, the matrix, when used in com bination with the ladder method of sequencing, allows the complete sequence of tens of picomoles of model oligodeoxynucleotides to be determined. (J A m Soc Mass Spectrom 2000, 11, 854-865) (C) 2000 American Society for Mass S pectrometry.