A RIBOSOMAL-RNA OPERON FROM PSEUDOMONAS-STUTZERI ZOBELL

A ribosomal RNA operon from the marine bacterium, Pseudomonas stutzeri Zobell, was cloned and characterized by Southern hybridization and se quence analysis. The 16S rRNA, 23S rRNA, 5S rRNA and 2 tRNA genes (ala nine and isoleucine) were identified by homology with sequences in Gen Bank. The rRNA gene exhibited typical eubacterial organization (16S-tR NAs-23S-5S). A putative ribosomal promoter and anti-terminator regions were also identified and described. Significant differences in spacin g of the anti-terminator regulatory elements were observed between P. stutzeri Zobell and Escherichia coli. (C) 1997 Elsevier Science B.V.