A study of turbidity in hypertriglyceridemic specimens

A study is presented that will investigate the role of severe turbidity in the form of hypertriglyceridemia on spectrophotometric measurements in solu tions. The aim is to see if such measurement parallels the effect of turbid ity on densitometry of electropherograms and thin-layer chromatography plat es as well as with reflectometry of reactions for clinically important mole cules in biological fluids such as serum. If this solution study does not p arallel measurement in solid mixtures, it seems reasonable to ask how varia bly diminished light relates to turbidity and how absorbance increases affe ct the measurements in a scattering medium. The principle of additivity in spectrophotometry would also be important in order for corrective blanking to be effective. Thus, the factors of a constant turbidity but with variabl e absorbances, the potential attendant ability to scatter light, decreasing in rising systems and increasing in regressing systems along with the prin ciple of additivity in spectrophotometry can be studied in this proposed in vestigation for solution measurement as compared to densitometry and reflec tometry. (C) 2000 Elsevier Science B.V. All rights reserved.