Immunological characterization of 130 kDa phospholipase C-beta 4 isozyme in rat cerebellar Purkinje cells

Authors
Min, DS Kim, MJ Jeong, HK Lee, YH Kim, H Shin, HS Ryu, SH Suh, PG
Citation
Ds. Min et al., Immunological characterization of 130 kDa phospholipase C-beta 4 isozyme in rat cerebellar Purkinje cells, NEUROSCI L, 292(1), 2000, pp. 9-12
Citations number
9
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE LETTERS
ISSN journal
03043940 → ACNP
Volume
292
Issue
1
Year of publication
2000
Pages
9 - 12
Database
ISI
SICI code
0304-3940(20000929)292:1<9:ICO1KP>2.0.ZU;2-1
Abstract
We have developed a monoclonal antibody, beta 4-22-9 that recognizes an epi tope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-beta 4 (PLC-beta 4). Tissue d istribution of 130 kDa PLC-beta 4 was determined with this monoclonal antib ody. One hundred and thirty kiloDalton PLC-beta 4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at ve ry high levels in cerebellum. The expression of 130 kDa PLC-beta 4 in cereb ellum was gradually increased from 5 days after birth to adulthood. in the adult cerebellum, especially high level of 130 kDa PLC-beta 4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data i mplies that 130 kDa PLC-beta 4 may a role in cerebellar Purkinje cells. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.