DNA electrophoresis in a monodisperse porous medium

Citation
L. Meistermann et B. Tinland, DNA electrophoresis in a monodisperse porous medium, PHYS REV E, 62(3), 2000, pp. 4014-4017
Citations number
14
Categorie Soggetti
Physics
Journal title
PHYSICAL REVIEW E
ISSN journal
1063651X → ACNP
Volume
62
Issue
3
Year of publication
2000
Part
B
Pages
4014 - 4017
Database
ISI
SICI code
1063-651X(200009)62:3<4014:DEIAMP>2.0.ZU;2-X
Abstract
Electrophoresis of DNA migrating in an ordered matrix is studied and compar ed with classical agarose gel electrophoresis. A well-defined migration med ium is obtained by crystallization of monodisperse silica spheres. Electrop horetic mobility of DNA is measured with fluorescence recovery after photob leaching experiments. The main result is that, as it was the case for gel e lectrophoresis, diffusion and electrophoretic mobility of DNA in such a med ium are well described with reptation theories.