W. Vilegas et al., Application of on-line C-30 RP-HPLC-NMR for the analysis of flavonoids from leaf extract of Maytenus aquifolium, PHYTOCH AN, 11(5), 2000, pp. 317-321
The application of on-line C-30-reversed-phase high-pressure liquid chromat
ography-nuclear magnetic resonance spectroscopy is described for the analys
is of tetraglycosylated flavonoids in aqueous and hydroalcoholic extracts o
f the leaves of Maytenus aquifolium (Celastraceae), Triacontyl stationary p
hases showed adequate separation for on-line IH-NMR measurements at 600 MHz
and allowed the characterisation of these flavonoids by detection of both
aromatic and anomeric proton signals, Copyright (C) 2000 John Wiley & Sons,
Ltd.