A. Tylicki et al., A new way of achieving plant regeneration of Solanum lycopersicoides Dun. from root primordia culture, PL CELL REP, 19(9), 2000, pp. 837-844
Cell aggregates with root primordia were formed in root primordia culture (
RPC) of Solanum lycopersicoides grown in modified liquid MS medium containi
ng 15 mg/l NAA. After transfer to liquid medium containing 1 mg/l 2,4-D, th
e aggregates dissociated into single root primordia (RP) which had an organ
izing root meristem at the apical pole. Oval structures called pseudoembryo
s were formed from single RP. After passage to liquid MS medium without phy
to-hormones and organic compounds (with the exception of sucrose), an apica
l root meristem developed and the shoot apical meristem was initiated. The
pseudoembryos developed into elongated pseudoseedlings which formed plants
after transfer to a 1/2MSV medium. The development of pseudoembryos occurre
d without the callus phase. Moreover, the induction of the shoot meristem o
ccurred without exogenous cytokinins.