Plant regeneration in Arachis pintoi (Leguminosae) through leaf culture

Plant regeneration in Arachis pintoi was obtained via two developmental pat hways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2 ,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration through organogenesis was an initial medium composed of 10 mg/l NAA+ 1 mg/l BA followed by transfer of the callus to a shoot induct ion medium (MS+1 mg/l BA. Rooting of regenerated shoots was readily achieve d by culture on MS+ (0.01 mg/l NAA. Embryogenic callus cultures were initia ted from pieces of leaf on MS medium supplemented with PICL in combination with KIN, ZEA. BA or 2iP, and the most suitable combinations were 10 mg/l P ICL+1 mg/l BA or 2iP. When pieces of embryogenic callus were subcultured on MS+1 mg/l EA, somatic embryos were differentiated and developed further in to well-developed plants in MS+1 g/l AC followed by MS medium devoid of pla nt growth regulators.