C and N fluxes of decomposing C-13 and N-15 Brassica napus L.: effects of residue composition and N content

The interactions occurring between biochemical composition and N content of crop residues while decomposing in soil, and the associated N dynamics wer e assessed by studying the kinetics of C and N biotransformations of differ ent tissues of Brassica napus L. (roots, stems and pod walls). These residu es were obtained by growing a rapeseed crop under low and high N nutrition, in a labeling growth chamber with enriched (CO2)-C-13 atmosphere and a N-1 5 nutritive solution. The resulting crop residues in which the C-to-N ratio varied between 22 and 135 were homogeneously labeled with C-13 and N-15. P aired labeled residues ((CN)-C-13-N-15 labeled residues with unlabeled soil inorganic N; (CN)-C-13-N-14 residues with N-15 labeled soil inorganic N) w ere used to determine net and gross fluxes of immobilization and mineraliza tion. Decomposition was studied during laboratory incubations at 15 degrees C, the initial soil N availability being non-limiting with regard to the r ate of C decomposition. The rate of C-13 mineralization from the residues w as influenced by the biochemical composition of the tissues and particularl y by their soluble C content. The N content of the tissues did not signific antly affect the kinetics or the amount of C mineralized, except in the ver y short-term Decomposition was rapid and after 168 days of incubation at 15 degrees C, 82% of the C from the stems and pod walls and 69% from the root s at both low and high N contents had disappeared from the soil coarse frac tion. Residue decomposition first resulted in net immobilization of soil mi neral N for all the residues. The intensity and duration of this immobiliza tion depended on the tissues and the N content of the residues. Compared to the control, the residues with low N content, still induced net N immobili zation after 168 days (-22 to -14 mg N g(-1) of added C) whereas the high N residues induced little net immobilization or mineralization, at -3 to + 4 mg N g(-1) of added C at the same date. The NCSOIL model was used as a too l to calculate, by fitting simulation against the data, the gross N mineral ization and immobilization fluxes and also to determine the total N fluxes involved over the 168 days of decomposition. Depending on the tissues and t heir N content, gross cumulative immobilization ranged from 71 to 113 mg N g(-1) of added C and gross mineralization Varied from 66 to 123 mg N g(-1) of added C. The differences in net mineralization, observed during decompos ition of the tissues with low and high N contents, were well explained by t he differences between gross mineralization fluxes which were themselves at tributable to the different quantities of N mineralized from the residues, The use of modeling to calculate the total gross N fluxes demonstrates that the total amount of N involved in the decomposition of crop residues is mu ch higher than the resulting net fluxes quantified either by N balance or b y N-15 tracing. (C) 2000 Elsevier Science Ltd. All rights reserved.