Interspecific genetic linkage map, segregation distortion and genetic conversion in coffee (Coffea sp.)

An interspecific partial genetic linkage map of Coffea sp. based on 62 back cross hybrids is presented. F-1 hybrids were generated by a cross between t he wild C. pseudozanguebariae and the anciently cultivated C. liberica var. dewevrei (DEW); progeny were then derived from a backcross between F-1 hyb rid and DEW. The map construction consisted of a two-step strategy using 5. 5 and 3.1 LOD scores revealed by simulation file. The map consisted of 181 loci: 167 amplified fragment length polymorphism (AFLP) and 13 random fragm ent length polymorphism (RFLP) loci. The markers were as sembled into 14 li nkage groups, each with 4-31 markers covering 1,144 cM. Segregation distort ion was observed for 30% of all loci, in particular 3:1 and 1:3 ratios equa lly favouring each of the two parents. The existence of such ratios suggest s genetic conversion events. This map also represents an initial step towar ds the detection of quantitative trait loci.