TRANSFORMATION OF BIFIDOBACTERIUM-LONGUM WITH PRM2, A CONSTRUCTED ESCHERICHIA-COLI B-LONGUM SHUTTLE VECTOR

Authors
MISSICH R SGORBATI B LEBLANC DJ
Citation
R. Missich et al., TRANSFORMATION OF BIFIDOBACTERIUM-LONGUM WITH PRM2, A CONSTRUCTED ESCHERICHIA-COLI B-LONGUM SHUTTLE VECTOR, Plasmid, 32(2), 1994, pp. 208-211
Citations number
7
Categorie Soggetti
Genetics & Heredity
Journal title
Plasmid → ACNP
ISSN journal
0147619X
Volume
32
Issue
2
Year of publication
1994
Pages
208 - 211
Database
ISI
SICI code
0147-619X(1994)32:2<208:TOBWPA>2.0.ZU;2-U
Abstract
An Escherichia coli-Bifidobacterium longum shuttle vector, designated pRM2, was constructed by cloning a B. longum plasmid and an enterococc al spectinomycin resistance gene into a commercial E. coli vector. The plasmid was successfully introduced into B. longum cells by electropo ration and into E. coli cells by both electroporation and chemical tra nsformation. (C) 1994 Academic Press, Inc,