An unmediated hydrogen peroxide sensor is designed in this paper by employi
ng a hemoglobin-SDS film modified electrode. Hemoglobin exhibits direct (un
mediated) electrochemistry at the modified electrode. The protein also show
s elegant catalytic activity towards the electrochemical reduction of hydro
gen peroxide. Consequently, a prototype hydrogen peroxide sensor is prepare
d. Under optimum conditions, this sensor provides a linear response over th
e hydrogen peroxide concentrations in the range of 1x10(-5)similar to 1x10(
-4) mol/L. The detection limit was 2x10(-6) mol/L The relative standard dev
iation was 4.2% for 6 successive determinations of the hydrogen peroxide at
1x10(-5) mol/L. This configuration is shown to be sensitive, stable and ea
sily fabricated. It might be useful in the biological and industrial fields
.