Although epilepsy often begins in childhood, factors that contribute to the
development of epilepsy as a consequence of status epilepticus (SE) during
early development are poorly understood. We investigated animal models in
which seizure-induced epileptogenicity could be studied. Rats undergoing se
lf-sustaining SE induced by perforant path stimulation (PPS) at the ages of
postnatal day 21 (P21) and P35 were compared with those subjected to SE by
lithium and pilocarpine (LiPC). Although only one animal subjected to PPS
at P21 developed chronic spontaneous seizures by several months of observat
ion, all the animals subjected to PPS at P35 became epileptic. In the LiPC
model, however, most of the rat pups subjected to SE at P21 became epilepti
c. Animals with spontaneous seizures showed increased inhibition in the den
tate gyrus, a characteristic of the epileptic brain, with evidence of mossy
fiber synaptic reorganization. Examination of circuit recruitment by c-Jun
immunohistochemistry showed activation restricted to the hippocampus in P2
1 animals subjected to PPS, although extensive activation of hippocampal an
d extrahippocampal structures was seen in pups subjected to PPS-induced sel
f-sustaining SE at P35 or LiPC SE at P21. These results demonstrate that th
e appearance of epilepsy as a consequence of SE is influenced by the type o
f insult as well as by age-dependent circuit recruitment.