Genetic relationships among Florida Diaprepes abbreviatus (Coleoptera : Curculionidae) populations

Genetic differentiation among six Florida populations of Diaprepes abbrevia tus (L.) was determined using protein and random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR) markers. Proteins were separated by el ectrophoresis and stained with silver stain and for alpha-naphthylacetate e sterase activity. No differentiation was observed among populations when eg g proteins were silver stained: alpha-naphthylacetate esterase activity dif ferentiated five of the six populations. RAPD-PCR data showed. significant differentiation among populations, consistent with the hypothesis of three independent introductions of D. abbreviatus into Florida. Our data indicate that D. abbreviatus populations, once introduced, have generally remained in one locality with limited dispersal to new areas.