In this study, an improved polymerase chain reaction (PCR) was used for det
ection of DNA of latent EHV-1 strains from several sources. Three pairs of
oligonucleotide primers spanning fragments of 333 bp, 226 bp and 268 bp of
the thymidine kinase (tk) gene, and one primer pair spanning 225 bp of the
glycoprotein C (gC) gene were used in specific amplifications. Primers for
EHV-4 PCR were also designed. Restriction digests with TaqI confirmed the i
dentity of tk PCR fragments from EHV-1. The sensitivity to detect PCR produ
cts was further improved by visualisation in silver-stained acrylamide gels
. PCR assays were applied to 267 samples including pools of tissue, periphe
ral blood leukocytes (PBL) and nasal swabs of archived, farms and abattoir
specimens from a total of 116 animals. The EHV-1 DNA was found in 88% of th
e analysed samples. The prevalence of the EHV-1 latent or persistent form i
n adult horses was similar to others reports but found higher than previous
ly described in foetuses and young foals. EHV-4 latency was not detected in
the Brazilian studied specimens.