Recognition of receptor lipopolysaccharides by spike G protein of bacteriophage phi X174

Authors
Kawaura, T Inagaki, M Karita, S Kato, M Nishikawa, S Kashimura, N
Citation
T. Kawaura et al., Recognition of receptor lipopolysaccharides by spike G protein of bacteriophage phi X174, BIOS BIOT B, 64(9), 2000, pp. 1993-1997
Citations number
21
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
9
Year of publication
2000
Pages
1993 - 1997
Database
ISI
SICI code
0916-8451(200009)64:9<1993:RORLBS>2.0.ZU;2-1
Abstract
The spike G protein of bacteriophage phi X174 was prepared as a hexa histid ine-tagged G protein (HisG). In the enzyme-linked plate assay, HisG bound s pecifically to lipopolysaccharides (LPSs) of the phi X174-sensitive strains , and did not bind to LPSs of the phi X174-insensitive strains. The truncat ed G protein obtained after trypsin digestion of HisG had the similar affin ity to the LPSs to HisG, indicating that eight amino acid residues from the N-terminus are not essential to the binding with the LPSs.