Y. Maruyama et T. Nakajima, The aman6 gene encoding a yeast mannan backbone degrading 1,6-alpha-D-mannanase in Bacillus circulans: Cloning, sequence analysis, and expression, BIOS BIOT B, 64(9), 2000, pp. 2018-2020
A gene (aman6) encoding endo-1,6-alpha-D-mannanase, a yeast mannan backbone
degrading enzyme from Bacillus circulans was cloned, The putative aman6 wa
s 1767 base pairs long and encoded a mature 1,6-alpha-D-mannanase protein o
f 589 amino acids and a signal peptide of 36 amino acids. The purified matu
re 1,6-alpha-D-mannanase from the Escherichia coli transformant showed 61-k
Da protein, and N-terminal amino acid sequence and other general properties
of the recombinant enzyme were identical to those of 1,6-alpha-D-mannanase
from Bacillus circulans TN-31.