Overexpression of an alkaline lipase gene from Proteus vulgaris K80 in Escherichia coli BL21/pKLE

Authors
Lee, HW Yoon, SJ Kim, HK Park, KM Oh, TK Jung, JK
Citation
Hw. Lee et al., Overexpression of an alkaline lipase gene from Proteus vulgaris K80 in Escherichia coli BL21/pKLE, BIOTECH LET, 22(19), 2000, pp. 1543-1547
Citations number
15
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
19
Year of publication
2000
Pages
1543 - 1547
Database
ISI
SICI code
0141-5492(200010)22:19<1543:OOAALG>2.0.ZU;2-Y
Abstract
Over-expression of Proteus vulgaris K80 lipase gene in Escherichia coli BL2 1 (DE3)/pKLE was achieved, with the enzyme being produced in an as active s oluble form, using the T7 RNA polymerase system in a modified M9 salt or M9 ZB medium. d-Lactose (55 mM) was used to induce gene expression and gave tw ice the lipase activity achieved with 0.4 mM IPTG. The expression of the li pase gene depended on the feeding rate of glucose being optimal at 12 g l(- 1) h(-1).