Adsorption of protein in the expanded bed

The influence of operation parameters on the adsorption performance of prot ein (bovin serum albumin, BSA) in an expanded bed was studied using Streaml ine diethyl aminoethyl (DEAE). The result of residence time distributions ( RTD) and breakthrough curves showed that adsorption performance of the expa nded bed could not be improved by increasing the flow velocity at the range from 16 ml.min(-1) to 26 ml.min(-1). The increase of protein concentration from 0.5 mg.ml(-1) to 2 mg.ml(-1) resulted in poor adsorption performance. With increasing temperature from 5 degrees C to 30 degrees C and the sedim ented bed height from 11.5 cm to 22.5 cm, the adsorption characteristics in the expanded bed were improved.