The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases

We report cloning and sequencing of gene psI encoding a versatile peroxidas e combining catalytic properties of lignin peroxidase (LiP) and manganese p eroxidase (MnP) isolated from lignocellulose cultures of the white-rot fung us Pleurotus eryngii. The gene contains 15 putative introns, and the deduce d amino acid sequence consists of a 339-residue mature protein with a 31-re sidue signal peptide. Several putative response elements were identified in the promoter region. Amino acid residues involved in oxidation of Mn2+ and aromatic substrates by direct electron transfer to heme and long-range ele ctron transfer from superficial residues as predicted by analogy with Phane rochaete chrysosporium MnP and LiP, respectively. A dendrogram is presented illustrating sequence relationships between 29 fungal peroxidases. (C) 200 0 Federation of European Microbiological Societies. Published by Elsevier S cience B.V. All rights reserved.