Identification of DNA amplifications near the center of the Streptomyces coelicolor M145 chromosome

Linear streptomycete chromosomes frequently undergo spontaneous gross DNA r earrangements at the terminal regions. Large DNA deletions of the chromosom e ends are in many cases associated with tandemly reiterated DNA amplificat ions, found at the border of the deletable areas. In contrast to previous r eports, we have discover-ed amplifications near the center of the Streptomy ces coelicolor M145 chromosome. The detected amplified units of DNA are 19. 9 kb and 16 kb in length and exist in copy numbers of 30 and 40, respective ly. Both amplifications were located in the same region and share at least 3.6 kb. (C) 2000 Federation of European Microbiological Societies. Publishe d by Elsevier Science B.V. All rights reserved.