Ozone (O-3) biomonitoring was conducted by means of the hypersensitive toba
cco cultivar Bel-W3 in the rural area of Collevalle (Central Italy) during
summer 1998. Four biomonitoring sites were set at different elevations and
distances from a nearby motorway. One additional biomonitoring site was est
ablished in the suburbs of the nearby town of Viterbo, a location where ins
trumental O-3 monitoring was continuously conducted since 1989, and phytoto
xic O-3 levels are known to occur. Passive O-3 monitoring was carried out b
y means of diffusive samplers. Biomonitoring with Bel-W3 tobacco showed tha
t O-3 pollution occurring in ambient air at Collevalle was comparable to th
at occurring at Viterbo. In situ validation against instrumental data sugge
sted that Bel-W3-based O-3 biomonitoring is superior to passive sampling in
terms of accuracy and sensitivity. Calibration against instrumental O-3 mo
nitoring allowed to obtain the first Bel-W3 tobacco dose-response curve for
the Viterbo area.