Three-dimensional surface reconstruction and fluorescent visualization of cardiac activation

Citation
Ma. Bray et al., Three-dimensional surface reconstruction and fluorescent visualization of cardiac activation, IEEE BIOMED, 47(10), 2000, pp. 1382-1391
Citations number
24
Categorie Soggetti
Multidisciplinary,"Instrumentation & Measurement
Journal title
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
ISSN journal
00189294 → ACNP
Volume
47
Issue
10
Year of publication
2000
Pages
1382 - 1391
Database
ISI
SICI code
0018-9294(200010)47:10<1382:TSRAFV>2.0.ZU;2-F
Abstract
Optical imaging of transmembrane potentials in cardiac tissue is a rapidly growing technique in cardiac electrophysiology. Traditional studies typical ly use a monocular imaging setup, thus limiting investigation to a restrict ed region of tissue, However, studies of large-scale wavefront dynamics, es pecially those during fibrillation and defibrillation, would benefit from v isualization of the entire epicardial surface. To solve this problem, a pan oramic cardiac visualization algorithm was developed which performs the two tasks of reconstruction of the surface geometry of the heart, and represen tation of the panoramic fluorescence information as a texture mapping onto the geometry that was previously created, This system permits measurement o f epicardial electrodynamics over a geometrically realistic representation of the actual heart being studied. To verify the accuracy of the algorithm, the procedure was applied to synthetic images of a patterned ball; further verification was provided by application of the algorithm to a model heart placed in the experimental setup, Both sets of images produced mean regist ration image errors on the order of 2 pixels, corresponding to roughly 3 mm on the geometry. We demonstrate the algorithm by visualizing epicardial wa vefronts on an isolated, perfused rabbit heart.