Modulation of collagen-induced arthritis by IL-4 and dexamethasone: the synergistic effect of IL-4 and dexamethasone on the resolution of CIA

We investigated effects of IL-4, dexamethasone (DXM), and the combination o f IL-4 and DXM, low- or high-dose, on collagen-induced arthritis (CIA) in D BA/1 mice and correlated severity of arthritis with changes in IL-10 and IF N-gamma. Compared with control mice, mice treated with IL-4 had increased I L-10 with the same degree of arthritis, whereas mice treated with high-dose DXM had decreased IL-10 and increased IFN-gamma production with less sever e arthritis. Mice treated with low-dose DXM showed the absence of IL-10 and increased IFN-gamma production with a trend toward the resolution of arthr itis. Mice treated with IL-4 and low-dose DXM had neither IL-10 nor IFN-gam ma production but revealed less severe arthritis, compared with mice treate d with low-dose DXM alone. These results suggest that the beneficial effect s of high-dose DXM and the combination of IL-4 and DXM on CIA are independe nt of IL-10 and IFN-gamma, (C) 2000 Elsevier Science B.V. All rights reserv ed.