Characterization of lipid pattern of Mycobacterium paratuberculosis isolates from goats and sheep

Lipid pattern of 18 wild type Mycobacterium paratuberculosis isolates were determined and compared with standard M. paratuberculosis (Teps) strain use d for commercial Johnin production. Fourteen of these isolates had typical pattern of alpha and keto mycolates in the ratio of 30: 70%, which resemble d the Lipid pattern of M. avium, therefore providing evidence of their iden tity as M. paratuberculosis. Additional band of methoxy mycolate present in the M. paratuberculosis (Teps) strain and 3 other isolates, suggested majo r differences in their identity as M. paratuberculosis. PDIM patterns of th ese isolates were also determined.