Genotyping of dairy animals using DNA from milk somatic cells

Authors
De, S Singh, RK Gupta, PK Palia, S Butchaiah, G
Citation
S. De et al., Genotyping of dairy animals using DNA from milk somatic cells, I J ANIM SC, 70(9), 2000, pp. 944-946
Citations number
9
Categorie Soggetti
Animal Sciences
Journal title
INDIAN JOURNAL OF ANIMAL SCIENCES
ISSN journal
03678318 → ACNP
Volume
70
Issue
9
Year of publication
2000
Pages
944 - 946
Database
ISI
SICI code
0367-8318(200009)70:9<944:GODAUD>2.0.ZU;2-3
Abstract
Genomic DNA was isolated from bovine milk somatic cells. Three milliliters (minipreps) and 30 mi (maxipreps) of milk samples were processed for isolat ion of genomic DNA. Use of this DNA for genotyping of cattle based on alpha -lactalbumin loci by polymerase chain reaction - restriction fragment lengt h polymorphism (PCR-RFLP) indicated that milk somatic cells can be an easy and reliable source of DNA for genotyping of farm animals or any other enzy matic reactions.