The status of thiaminThe status of thiamin (B-1), riboflavin (B-2), ascorbi
c acid (AA), and tocopherol was determined in 60 leisure athletes (age 46 /- 10 y, BMI 23.7 +/-2.0 kg.m(-2), VO2max 39.4 +/- 6.5 ml.min(-1).kg(-1)),
who completed a 100-km walking race. Vitamin plasma levels and activities o
f erythrocyte transketolase (ETK) and glutathione reductase were measured b
efore start, immediately after finishing and 6 hours later. The participato
rs finished entire distance in 14.25 h (average speed 7 km.h(-1)). Before s
tart. all participators showed an excellent vitamin status (prevalences of
low vitamin status ranged between 1.7 and 1.8%). Plasma tocopherol concentr
ations correlated significantly with increased age (r = 0.35, p = 0.008). C
ompared to the values before start, plasma concentrations of B-1 and B-2 as
well as ETK and/or EGR were increased significantly after finishing. The T
he raised levels persisted 6 hours after finishing, whereas AA remained una
ltered. The univariate analysis of variance revealed;that the change in vit
amin status after finish and 6 hours later was in part highly dependent on
age, BMI and the level of physical fitness. Despite the long distance. the
extensive character of the 100-km walking with its low intensity did not de
teriorate the measured indicators of vitamin status.