Experimental study on the possibility of treatment of some hemorrhagic fevers

Citation
G. Ignatyev et al., Experimental study on the possibility of treatment of some hemorrhagic fevers, J BIOTECH, 83(1-2), 2000, pp. 67-76
Citations number
38
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOTECHNOLOGY
ISSN journal
01681656 → ACNP
Volume
83
Issue
1-2
Year of publication
2000
Pages
67 - 76
Database
ISI
SICI code
0168-1656(20000929)83:1-2<67:ESOTPO>2.0.ZU;2-F
Abstract
After intracerebral challenge with 100 PFU of Lassa virus (strain Josiah), all infected mice (CBA/calac) died (control group). Production of pro-infla mmatory cytokines (IL-1 beta, TNF-alpha) significantly increased in the blo od of these Nice during the infection. For neutralization of increasing con centrations of these cytokines recombinant IL-IRA was used intraperitonealy at a dose 100 mu g kg(-1), everyday, within 5 days from the third day afte r the challenge. Injections of IL-IRA decreased the concentration of IL-1 b eta and TNF-alpha and resulted in survival of all infected mice (treatment group). Marburg fever (strain Popp) caused in guinea pigs by 5 LD50 of viru s lead to the significant increase of TNF-alpha in the animal's blood and c aused a lethal outcome (control group). Treatment of infected guinea pigs b y IL-IRA or anti-TNF serum decreased the concentration of TNF-alpha and res ulted in survival of half of the animals (treatment group). For the treatme nt recombinant IL-IRA was used at a dose 100 mu g kg(-1) intramuscularly, e veryday, within 6 days from the third day after the challenge or anti-TNF s erum, intramuscularly 0.5 mi (2000 U ml(-1); 1 U of the antiserum neutralis es 0.03 ng of TNF-alpha), everyday, within 6 days from the third day after the challenge. (C) 2000 Elsevier Science B.V. All rights reserved.