Interaction between inflammatory cells and heparin-surface-modified intraocular lens

Purpose: To investigate the interaction and adherence of inflammatory cells to a heparin-surface-modified intraocular lens (HSM IOL). Setting: Department of Ophthalmology, Tokyo Medical University Hospital, To kyo, Japan. Methods: Splenic mononuclear leukocytes from rats with experimental autoimm une uveitis were cultured with the optic of an HSM IOL for 96 hours. The nu mber of adherent cells on the HSM IOL surface was measured with and without the addition of interphotoreceptor retinoid-binding protein and concanaval in A (ConA) to the culture medium. The adherent cells were observed under a light microscope or a scanning electron microscope. Results: Interphotoreceptor retinoid-binding protein and Con, increased the number of adherent cells on the HSM IOL relative to the control. Adherent cells on the HSM IOL were small and round, considered to be mainly lymphocy tes. Conclusion: Activated lymphocytes tended to adhere to the surface of the HS M IOL. (C) 2000 ASCRS and ESCRS.