Use of amplified fragment length polymorphisms for typing Corynebacterium diphtheriae

Citation
A. De Zoysa et A. Efstratiou, Use of amplified fragment length polymorphisms for typing Corynebacterium diphtheriae, J CLIN MICR, 38(10), 2000, pp. 3843-3845
Citations number
14
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
38
Issue
10
Year of publication
2000
Pages
3843 - 3845
Database
ISI
SICI code
0095-1137(200010)38:10<3843:UOAFLP>2.0.ZU;2-9
Abstract
Amplified fragment length polymorphism (AFLP) was investigated for the diff erentiation of Corynebacterium diphtheriae isolates. Analysis using Taxotro n revealed 10 distinct AFLP profiles among 57 isolates. Strains with riboty pe patterns D1, D4, and D12 could not be distinguished; however, the techni que discriminated isolates of ribotype patterns D3, D6, and D7 further. AFL P mas rapid, fairly inexpensive, and reproducible and could be used as an a lternative to ribotyping.