cDNA cloning of a human RAB26-related gene encoding a Ras-like GTP-bindingprotein on chromosome 16p13.3 region

Members of the RAB protein family are important regulators of vesicular fus ion and trafficking. A putative new member of the RAB family of genes was i dentified through a public database search, and its full-length cDNA was is olated from a human fetal brain cDNA library. The predicted protein product of the gene consists of 190 amino acid residues and has 87% identity with rat Rab26. Thus, we designated this gene as the human RAB26-related gene. R everse transcription-coupled polymerase chain reaction (RT-PCR) demonstrate d that the RAB26-related messenger RNA was predominantly expressed in adult and fetal brain. Furthermore, an RT-PCR experiment for brain subregions sh owed that the mRNA was highly expressed in the amygdala, cerebellum caudate nucleus, and hippocampus. By PCR-based analysis with both a human/rodent m onochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 16p13.3 region between markers WI-7742 and WI-306 1. The RAB26-related gene consists of eight exons that span about 44kb of t he genome DNA.