Characterization of a new spotted fever group rickettsia detected in Ixodes ricinus (Acari : Ixodidae) collected in Slovakia

Two previously undescribed rickettsiae were detected in Ixodes ricinus Rick etts by polymerase chain reaction. ixodes ricinus Slovakia (IR) 3 and IRS4 were identified in the ticks collected in northeastern and southwestern Slo vakia, respectively. Sequences of the 16S rRNA citrate synthase (gltA) and outer membrane protein rOmpA (ompA) encoding genes of both strains were nea rly identical but were distinct from those of all other known rickettsiae. Phylogenetic relationships inferred from the comparison of these sequences with those of other members of the genus Rickettsia indicate that IRS3 and IRS4 constitute a new rickettsial genotype and form a separate cluster amon g the spotted fever group rickettsiae.