Mh. Weng et al., Failure of dengue-2 virus antibody to interfere with the isolation of dengue-2 virus from Aedes aegypti (Diptera : Culicidae), J MED ENT, 37(4), 2000, pp. 641-644
When isolating dengue virus (DEN) from mosquitoes collected in endemic area
s, pools may contain both anti-dengue antibodies from freshly engorged fema
les and virus from DEN infected females. To determine if these antibodies m
a) interfere with virus isolation, we simulated the isolation procedure usi
ng Aedes aegypti (L.) that we infected with the 16681 strain of dengue type
2 virus by intrathoracic inoculation. At 7 d postinfection, we allowed fem
ales to engorge on immunized or normal mouse blood. Virus in a mixture of a
nti-dengue-e antibodies and dengue-2 virus became inactive after incubation
at 37 degrees C for 1 h, but remained infective without incubation. Theref
ore, at ambient conditions antibodies would not interfere with virus isolat
ion from field-collected Ac, aegypti from endemic areas. In addition, DEN a
ntibodies enhanced virus replication when inoculated into Ae. aegypti, but
not C6/36 cells. The mechanism for this in vitro antibody enhancement of in
fection remains unclear.